Journal: Physics and Imaging in Radiation Oncology

Article Title: Imaging the tumour microenvironment in rectal cancer: Decline in tumour blood flow during radiotherapy predicts good outcome

doi: 10.1016/j.phro.2023.100417

Figure Lengend Snippet: a) Correlation between the mri-assessed tumourblood flow (BF) after treatment and the percentage endothelial marker CD34 staining in the excised tumour specimen. Spearman’s orrelation coefficient (ρ) and p-value are indicated. b) CD34 staining of two different patients with 1.5% staining (left) and 10.6% staining (right). CD34 highlights the endothelial cells in blood vessels, showing strong staining. There is some weak unspecific background staining in the epithelial cells.

Article Snippet: Counterstaining was performed with Hagens hematoxylin (diluted 1:4) for 5 min. Staining with CD34 (mouse monoclonal antibody, clone QBEnd/10, Nordic BioSite, Sweden) was performed with the Autostainer Link (Dako), using the Dako Envision Flex Code 8000 visualization kit.

Techniques: Marker, Staining

Journal: BMC Cancer

Article Title: Correlation between CD105 expression and postoperative recurrence and metastasis of hepatocellular carcinoma

doi: 10.1186/1471-2407-6-110

Figure Lengend Snippet: A , Positive staining for CD105 in HCC tissue (avidin-biotin-peroxidase complex, original magnification × 200). B , Negative staining for CD105 in paracarcinomatous liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). C , Negative staining for CD105 in normal liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). D , Positive staining for CD34 in HCC tissue (avidin-biotin-peroxidase complex, original magnification × 200). E , Positive staining for CD34 in paracarcinomatous liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). F , Positive staining for CD34 in normal liver tissue (avidin-biotin-peroxidase complex, original magnification × 200).

Article Snippet: Antigen retrieval was performed by first pretreating sections in a microwave oven or incubating them in 0.1% trypsin for 10 minutes at 37.0°C, after which nonspecific immunoreactivity was blocked by incubating the tissue in normal goat serum at room temperature for 5 minutes before application of the mAb to CD105(clone SN6h, NEOMARKERS), mAb to CD34(clone QBEnd/10, NEOMARKERS), or mAb to VEGF(clone JH121, NEOMARKERS), which were diluted with phosphate-buffered saline (PBS; 0.01 M, pH7.2) at a ratio of 1:100 for 1 h at 37°C.

Techniques: Staining, Avidin-Biotin Assay, Negative Staining

Journal: BMC Cancer

Article Title: Correlation between CD105 expression and postoperative recurrence and metastasis of hepatocellular carcinoma

doi: 10.1186/1471-2407-6-110

Figure Lengend Snippet: Correlation between CD34-MVD and CD105-MVD.

Article Snippet: Antigen retrieval was performed by first pretreating sections in a microwave oven or incubating them in 0.1% trypsin for 10 minutes at 37.0°C, after which nonspecific immunoreactivity was blocked by incubating the tissue in normal goat serum at room temperature for 5 minutes before application of the mAb to CD105(clone SN6h, NEOMARKERS), mAb to CD34(clone QBEnd/10, NEOMARKERS), or mAb to VEGF(clone JH121, NEOMARKERS), which were diluted with phosphate-buffered saline (PBS; 0.01 M, pH7.2) at a ratio of 1:100 for 1 h at 37°C.

Techniques:

Journal: BMC Cancer

Article Title: Correlation between CD105 expression and postoperative recurrence and metastasis of hepatocellular carcinoma

doi: 10.1186/1471-2407-6-110

Figure Lengend Snippet: Postoperative overall survival rate of patients with HCC. The comparison shown is according to the MVD determined with an anti-CD105 mAb A or an anti-CD34 mAb B .

Article Snippet: Antigen retrieval was performed by first pretreating sections in a microwave oven or incubating them in 0.1% trypsin for 10 minutes at 37.0°C, after which nonspecific immunoreactivity was blocked by incubating the tissue in normal goat serum at room temperature for 5 minutes before application of the mAb to CD105(clone SN6h, NEOMARKERS), mAb to CD34(clone QBEnd/10, NEOMARKERS), or mAb to VEGF(clone JH121, NEOMARKERS), which were diluted with phosphate-buffered saline (PBS; 0.01 M, pH7.2) at a ratio of 1:100 for 1 h at 37°C.

Techniques:

Journal: BMC Cancer

Article Title: Correlation between CD105 expression and postoperative recurrence and metastasis of hepatocellular carcinoma

doi: 10.1186/1471-2407-6-110

Figure Lengend Snippet: Multivariate analysis of prognostic factors in hepatocellular carcinoma

Article Snippet: Antigen retrieval was performed by first pretreating sections in a microwave oven or incubating them in 0.1% trypsin for 10 minutes at 37.0°C, after which nonspecific immunoreactivity was blocked by incubating the tissue in normal goat serum at room temperature for 5 minutes before application of the mAb to CD105(clone SN6h, NEOMARKERS), mAb to CD34(clone QBEnd/10, NEOMARKERS), or mAb to VEGF(clone JH121, NEOMARKERS), which were diluted with phosphate-buffered saline (PBS; 0.01 M, pH7.2) at a ratio of 1:100 for 1 h at 37°C.

Techniques: